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Femiint Fertility

IVF success rates: How Air quality in the IVF Lab influences outcomes

One of the most significant factors which bring fruition to IVF is the Air Quality inside the IVF lab.  For this reason, at Femiint Health, after thorough research, a state-of-the-art IVF laboratory has been built with one of the best Modular Clean Rooms of globally recommended standards.
 
An embryo’s development is influenced by the quality of air in the lab.  Embryos breathe (absorb) air just like we do.  They do not yet have a liver, spleen, and kidneys to filter out pollutants in the air.  In an IVF lab, the embryos in the petri dish have no protection and are in direct contact with the air that we breathe.
 
Air has compounds such as chemical air contaminants (CACs), volatile organic compounds (VOCs), or polychlorinated biphenyls (PCBs), which could easily end up in the petri dish or settle on surfaces or dissolve in aqueous solutions of the embryo culture medium and even more so in the lipophilic oil overlays via body perfumes, cleaning solutions, glues, paint fumes, off-gassing from certain flooring, exhaust in the parking lot, or general air pollution.  Just a short exposure to any of these compounds can irreversibly damage or kill embryos.
 
At Femiint Health, the IVF laboratory is equipped with a specialized 5-stage air filtration system with clear separation of clean and dirty flow channels, automated hermetically sealed doors with pass boxes (for material traffic between the operation theatre and IVF lab) and a separate air handling unit for each room in the IVF lab.
Clean Room Technology
 
A clean room is a contained space with provisions to maintain the rate of filtration of particles at a higher level than the rate of contamination per minute.  They are classified based on the number and sizes of particles permitted per volume of air.  For an IVF lab, Class-10000 variety is recommended.
 
Clean room environment is achieved by highly filtered air being flushed through High Efficiency Particle Air (HEPA) filters under positive pressure.  The filters are housed within ducts that use chemical/absorbent filtration and/or UV light irradiation, photocatalytic conversion or a combination of some or all these methods.
At Femiint Health, validation of air quality is done periodically.  Our embryologist calculates the key performance indicators such as in vitro fertilization rate, cleavage rates, embryo quality and pregnancy rates on a quarterly basis to ensure that the laboratory is running as expected.
 
It is mandatory that all furniture, cleaning methods, and clothing are clean room approved.  The laboratory should have smooth, non-porous walls, impervious unbroken surfaces with corners and ledges that are easy to clean, no sliding doors, sinks or drains.  Cladding with non-porous materials such as panels made of aluminium tri-hydrate provide an inert (low VOC), hypoallergenic, and easy to clean wall.  Lighting should be within sealed lighting units to reduce airborne particles.
 
Ducts generally tend to get clogged with dust and debris and also have high humidity and a high propensity for mould (Fungi).  To prevent this, the ducts and pipes can be insulated internally by hiding them between wall panels or externally by covering them within an alcove.  At Femiint, we have given paramount importance to air ducts & pipes by coating them internally with antifungal & antibacterial materials to prevent mould.
 
A non-compromising attitude towards maintaining air quality at Femiint Health is a reason why we are ranked as One of the Top fertility clinic in Bangalore with a high success rate for IVF procedure.

Everything that you should know about In Vitro Fertilization or IVF

In Vitro Fertilization (IVF) is a form of Assisted Reproductive Technology or ART. It is a process of fertilization where matured eggs extracted from the ovary are combined with a sperm sample. It is a manual process carried out in test tubes within laboratories. The IVF process involves fertilizing the egg outside of the body and later implanting the embryo into the uterus for conception.
 
Steps In IVF Procedure:
 
Ovulation Induction:
Ovulation induction is a fertility treatment that is administered by way of medication to induce ovulation to encourage the development and release of eggs from the ovary. In case the subject is using her own eggs during IVF, she would then require commencing treatment with synthetic hormones to stimulate her ovaries to produce multiple eggs rather than a single egg that is produced every month. The need for multiple eggs arises as not all eggs fertilize or develop normally for fertilization.
A subject might need the following medications for inducing ovulation;
 
Medication for Ovarian Stimulation:
An injectable medication containing follicle stimulating hormone (FSH) or luteinizing hormone (LH) or combination of both is administered to the subject to stimulate ovaries to produce more than one egg at a time.
 
Oocyte Maturation:
A medication named human chorionic gonadotropin (HCG) is administered to the subject after 8 to 12 days, when the follicles are ready for egg or oocyte retrieval. This is done to help the eggs mature.
 
Prevent Premature Ovulation:
These medications are administered to prevent the body from releasing premature eggs.
 
Prepare the Lining of Uterus:
The lining of the uterus is an important factor for IVF. The thickness of the lining of uterus also termed endometrium is essential for retrieving eggs or successfully implanting the embryo. In instances where the lining is not receptive and thick enough for implantation, doctors may suggest the use of a medication called progesterone to make the lining of the uterus thicker and more receptive.
 
Egg Retrieval:
The process of egg or oocyte retrieval is carried out 34 to 36 hours after the subject has been administered human chorionic gonadotropin (HCG) and before ovulation.
Doctors before carrying out the process of egg retrieval sedate the subject to minimize pain.
The eggs are retrieved from the subject through transvaginal aspiration meaning the process or retrieving eggs from the ovary. An ultrasound supervised probe is inserted into the vagina to identify the follicles. A thin needle is then inserted through an ultrasound guide that penetrates the vagina and into the follicles to retrieve the eggs.
 
In instances where the ovary is not accessible through transvaginal aspiration, an abdominal surgery or laparoscopy may be advised. Abdominal surgery or laparoscopy also known as key hole surgery is carried by making a small incision around the navel. A viewing instrument is then inserted through the incision to help guide the needles to the follicles to retrieve eggs.
Eggs are retrieved from the follicles using suction.
Immediately after retrieving the eggs from the ovary they are placed in a special solution also called insemination media or culture medium. Insemination media or culture medium is a nutritive solution that provides the retrieved eggs with the required nutrition to maximize the likelihood of successful fertilization of eggs by the sperm.
 
Sperm Retrieval:
The partner of the subject provides for a semen sample for the sperm to be extracted. The semen sample is required in the morning when the egg is retrieved. For men who do not ejaculate the sperm can be directly retrieved from the testis using a surgical procedure. Donor sperms can also be utilized for the purpose.
 
Fertilization:
There are two common methods used for fertilization
 
Insemination:
In this method the healthy sperm and matured eggs are mixed and incubated overnight.
 
Intracytoplasmic sperm injection (ICSI):
This method of fertilization is used when the semen quality or the number is a problem or in case of failed fertilization attempts in previous IVF cycles. ICSI requires the healthy sperm to be directly injected into each mature egg.
 
Embryo Development:
Embryo development or embryogenesis is the process by which the assimilation of sperm and mature egg develops into an embryo.
 
Assisted Hatching:
In many instances of IVF treatment, the process fails because the embryo is unable to implant in the uterus. The embryo detaches from the outside membrane called the zona pellucida after 5 to 6 days of fertilization. Immediately after hatching the embryo implants itself in the uterus. For women who have experienced unsuccessful IVF attempts, doctors recommend assisted hatching. Assisted hatching is a technique by which a hole is made in the zona pellucida just before transfer for the embryo to successfully hatch and implant in the uterus.
 
Preimplantation Genetic Testing or PGD:
PGD is a procedure carried out before implantation of an embryo to check if the embryo has any defects. This is done to ensure that no genetic disorders or abnormalities are passed on to the child. While PGD is carried out as a preventive measure, it cannot be considered a safeguard.
 
Embryo Transfer:
Embryo transfer pertains to the transferring of embryos into a woman’s uterus to encourage pregnancy. The process takes place after 2 to 5 days from egg retrieval.
The subject is administered mild sedatives and may experience mild cramping
A long flexible tube called a catheter is inserted into the woman’s vagina, running through the cervix into her uterus.
A syringe containing one or more embryos is attached to the catheter and is transferred to the woman’s uterus by the doctor.
 
Embryo Cryopreservation:
Embryo Cryopreservation or embryo freezing is a process carried out by most IVF programs. It is a process by which healthy embryos are stored at sub zero temperatures for use later. Women who fail to conceive in earlier attempts can use these embryos for pregnancy instead of going through the complete IVF cycle. It can also be utilized for embryo donation for women wishing to conceive.
 
After the Procedure
While women can carry on with their day to day routine it is advised that after the implantation of embryos women do not over exert themselves physically.
 
Side Effects
Discharge of small amounts of clear or bloody fluid can occur owing to the swabbing of the cervix before the transfer of embryo.
High estrogen levels may cause breast tenderness.
Mild bloating or cramping may exist.
Constipation
In cases of severe pain after the embryo transfer subject is advised to visit the doctor to negate complications such as infection, twisting of an ovary and severe hyper stimulation syndrome.

Fertility Treatment – Myth versus Fact

Have you have been trying to conceive unsuccessfully? Have you had unprotected sex for over a year, with no results? Are you worried about conception because one or both of you are over 35 years?
You need expert advice.
Femiint Fertility at Whitefield which is rated as one of Bangalore’s best IVF Fertility Treatment Centres owing to its high success rate has a specialised team of doctors and staff who will be happy to walk you through your questions, however small they might appear to you.
Let’s explore some common myths and facts regarding infertility and fertility treatment.
Six Myths about Infertility and Fertility Treatment:
Myth No 1: Fertility is not an issue for men and women below 40 years
Age does have an effect on both men and women.
Women are born with an ova bank of around 10 to 20 lakh eggs. These eggs will not increase. Every month they lose about eleven thousand eggs before puberty, by which time they are left with about 3-4 lakh eggs. Subsequently about a thousand eggs are inevitably destroyed every month. By the time they are in their late twenties the numbers have drastically dwindled. The quality and quantity decrease, with each passing year.
Men over the age of 40 begin to have a decline in the quantity and quality of sperm that is produced.
Myth No 2: Being young and living a healthy lifestyle is a sure sign of being fertile
Healthy living is a key to living a full life and increases the fertility index. However, healthy living is not a guaranteed indicator of fertility. A healthy man who can ejaculate is not necessarily fertile. Semen is an organic fluid that may or may not have spermatozoa (sperm). A normal ejaculation may contain millions of sperms. But men can also have no sperms or a low sperm count. The motility of the sperms as well as the quality of sperms is directly linked to fertility.
Even for healthy women, one in ten below the age of 30 years is infertile. This percentage increases with age.
Myth No 3: A failure to conceive is because the woman is infertile in the majority of couples
This is not true. Studies have shown that 1/3rd of the problems with infertility are related to the male, and 1/3rd to the woman. The remaining 1/3rd is related to unknown causes.
Myth No 4: IVF does not necessarily result in conception or may result in more than one child
IVF treatment is a reliable procedure which most often gives positive results. The IVF treatment cycles are planned around the woman’s ovulation cycle. Advanced technology usually ensures the formation of a single or multiple embryo. With expert consultation, the chance for failure is minimal.
Myth No 5: As long as one has a healthy sex life, one can keep on trying for a child
A healthy sex life is not a precursor to pregnancy. There could be issues faced by either partner.
Delaying treatment can be costly. Besides, the success rate is better with age.
Myth No 6: Having one Baby ipso facto means one is not infertile
Secondary infertility which is the inability to conceive after the first child is a reality. This can happen due to many reasons, from either partner, such as low production of sperm in men, blocked fallopian tubes in women etc.
If you are struggling with secondary infertility, don’t despair. There is hope. Consult a Fertility Specialist straightaway.
Femiint Fertility is a specialised IVF treatment Center at Femiint Health Hospital, Whitefield, Bangalore. Give them a call on 080-4931-1111 to fix an appointment with a Fertility Specialist. At Femiint Health, IVF treatment costs are affordable and the treatment is personalized. You could soon be on your way to bringing home a bundle of joy.
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